Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
Number of patents in Portfolio can not be more than 2000
United States of America
Stats
-
Jul 30, 2024
Grant Date -
N/A
app pub date -
Mar 14, 2019
filing date -
Mar 14, 2019
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The present invention relates to methods and products for localized or spatial detection and/or analysis of RNA in a tissue sample or a portion thereof, comprising: (a) providing an object substrate on which at least one species of capture probe, comprising a capture domain, is directly or indirectly immobilized such that the probes are oriented to have a free 3′ end to enable said probe to function as a reverse transcriptase (RT) primer; (b) contacting said substrate with a tissue sample and allowing RNA of the tissue sample to hybridise to the capture probes; (c) generating cDNA molecules from the captured RNA molecules using said capture probes as RT primers; (d) labelling the cDNA molecules generated in step (c), wherein said labelling step may be contemporaneous with, or subsequent to, said generating step; (e) detecting a signal from the labelled cDNA molecules; and optionally (f) imaging the tissue sample, wherein the tissue sample is imaged before or after step (c).
First Claim
Other Claims data not available
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
- 10 Citation Count
- C12Q Class
- 98.33 % this patent is cited more than
- 1 Age
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 3.5 Year Payment | $1600.00 | $800.00 | $400.00 | Jan 30, 2028 |
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | Jan 30, 2032 |
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Jan 30, 2036 |