Mouse cell line authentication
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United States of America Patent
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Feb 13, 2024
Grant Date -
N/A
app pub date -
Jan 31, 2019
filing date -
Jul 3, 2013
priority date (Note) -
In Force
status (Latency Note)
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Abstract
A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (STR) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each STR marker. Correlations between allele fragment length and repeat number were determined with DNA Sanger sequencing. Genotypes for L929 and NIH3T3 cell lines were shown to be stable with increasing passage numbers as there were no significant differences in fragment length with samples of low passage when compared to high passage samples. In order to detect cell line contaminants, primers for two human STR markers were incorporated into the multiplex assay to facilitate detection of human and African green monkey DNA. This multiplex assay is the first of its kind to provide a unique STR profile for each individual mouse sample and can be used to authenticate mouse cell lines.
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International Classification(s)
- 2019 Application Filing Year
- C12Q Class
- 3899 Applications Filed
- 1757 Patents Issued To-Date
- 45.07 % Issued To-Date
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- C12Q Class
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Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 3.5 Year Payment | $1600.00 | $800.00 | $400.00 | Aug 13, 2027 |
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