Solution phase homogeneous assays
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United States of America Patent
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Mar 21, 2023
Grant Date -
N/A
app pub date -
Jul 31, 2020
filing date -
Feb 27, 2009
priority date (Note) -
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status (Latency Note)
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Abstract
Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
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