DNA vector production system
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United States of America Patent
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Nov 15, 2022
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N/A
app pub date -
Mar 5, 2020
filing date -
Nov 22, 2012
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Abstract
A vector production system is provided. The system comprises recombinant cells designed to encode at least a first recombinase under the control of an inducible promoter and the cells include an expression vector encoding a nucleic acid of interest within the regulatory elements of the expression vector which are flanked on either side by a target sequence for at least the first recombinase. The vector production system provides an efficient one-step process for producing linear or circular covalently closed vectors that incorporate a nucleic acid sequence of interest.
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