Recombinant microorganisms and uses therefor
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United States of America Patent
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Jun 12, 2018
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May 30, 2013
filing date -
May 30, 2012
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Abstract
Bacteria are genetically engineered to produce 3-hydroxypropionate (3-HP). The bacteria are carboxydotrophic acetogens. The bacteria produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/CO2. A malonyl-coA reductase from a bacterium that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced The production of 3-HP can be improved by overproduction of acetyl-CoA carboxylase or by overproduction of biotin. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.
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