Methods for reducing aggregate levels in protein preparations by treatment with thio-heterocyclic cations
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United States of America Patent
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Jun 5, 2018
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Feb 5, 2014
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Feb 6, 2013
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Abstract
A method of reducing the aggregate content in a protein preparation having a target protein includes contacting the protein preparation with a thio-heterocyclic cation to form a mixture, contacting the mixture with at least one functionalized solid to remove excess thio-heterocyclic cations; and optionally contacting the mixture, simultaneously or sequentially, with at least one further functionalized solid to further reduce aggregate content of the protein preparation.
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