Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
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United States of America Patent
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May 29, 2018
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Feb 26, 2015
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Feb 27, 2014
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Abstract
A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.
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