Process for preparation of sugars and syrups
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United States of America Patent
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May 29, 2018
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app pub date -
Apr 10, 2014
filing date -
Apr 10, 2013
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Abstract
The present invention relates to a process for producing a syrup comprising liquefying an aqueous granular starch slurry with an alpha-amylase variant comprising an alteration at one or more positions corresponding to any of positions 1, 2, 68, 71, 126, 133, 142, 144, 156, 158, 176, 185, 201, 205, 213, 239, 279, 316, 318, 360, 416, 437 and 450 of SEQ ID NO: 1 to provide liquefied starch-containing material; saccharifying the liquefied starch-containing material in the presence of a glucoamylase, and a pullulanase derived from Bacillus deramificans, Bacillus subtilis, Bacillus amyloderamicans, or Bacillus acidopullulyticus to provide a dextrose syrup, and optionally isomerizing to provide a fructose syrup.
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