Select on of extracellular matrix components and or matricellular proteins for improved post-cryopreservation cell viability and retention
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United States of America Patent
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May 22, 2018
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app pub date -
Oct 24, 2014
filing date -
Oct 24, 2014
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Abstract
A method for cryopreservation of adherent cells attached to a substrate and a method for identifying one or more extracellular matrix (ECM) components and/or matricellular proteins that improves viability and retention of the cryopreserved cells on the substrate. The method for cryopreservation includes treating a substrate with at least one ECM component and/or adding at least one matricellular protein to the substrate or cell culture media, plating the cells on the treated substrate, and cryopreserving the cells. One or more ECM components and/or matricellular proteins that improves cell viability and retention can be identified by evaluating the cells that have been thawed from the cryopreservation temperature to determine cell viability and retention.
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