Enzymatic hydrolysis of acylated steryl glycosides and method for treating biofuel
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United States of America Patent
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May 1, 2018
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Aug 13, 2014
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Aug 16, 2013
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Abstract
The present invention relates to the use of at least one glycosidase of the class EC 3.2.1.21, EC 3.2.1.4, EC 3.2.1.7, and/or EC 3.2.1.80 for hydrolyzing at least one glycosiding bond in at least one substrate selected from the group of acylated steryl glycosides of the general formula (I): R1—CO—O—[CmHnOx]y—O—R2 wherein R1 is a saturated or non-saturated C1-C30 alkyl, R2 is a sterol moiety, m=5-7, n=9-14 and x=5-7, y=1-5. The present invention relates further to a method of for hydrolysing steryl gylcosides using said enzymes in biofuel, oil and/or fat.
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