Method for the specific isolation of nucleic acids of interest
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Apr 17, 2018
Grant Date -
Nov 26, 2015
app pub date -
Jan 27, 2014
filing date -
Jan 25, 2013
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A method and diagnostic kit for selective isolation of microorganisms of interest and/or nucleic acids of interest including: a) bringing a liquid biological sample into contact with a saponin formulation to destabilize untargeted elements within the liquid biological sample, b) inducing osmotic shock of the untargeted elements to specifically lyse the untargeted elements, c) adding a solution of at least one enzyme capable of lysing free nucleic acids derived from the untargeted elements lysed in solution in the sample, and d) selectively obtaining the microorganisms of interest or the nucleic acids of interest. A precipitant of the unlyzed microorganisms of interest may be optionally added in solution. The microorganisms of interest have cell membranes or capsids that do not contain cholesterol. The untargeted elements may include untargeted cells having cell membranes containing cholesterol, and optionally viruses with envelopes containing cholesterol, mycoplasmas containing cholesterol, and cell debris.
First Claim
Family
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully