Method of expressing goldfish gfTP1 tranposase protein
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Apr 17, 2018
Issued Date -
N/A
app pub date -
Jan 4, 2015
filing date -
Mar 24, 2014
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
An expression method of active goldfish gfTP1 transposase protein comprises: constructing an expression vector comprising a gfTP1 transposase reading frame of a goldfish Tgf2 transposon, after transferred into escherichia coli Rosetta 1 (DE3), culturing an expression strain until absorbance of a bacteria solution under OD600 reaches 0.3-0.4, adding IPTG, culturing under 21-22° C. in shaking of 150-200 rpm, inducing to express soluble recombinant protein, and purifying to obtain a functionally active transposase. Also provided are an expression plasmid and the expression strain of the goldfish gfTP1 transposase protein, and a use of the strain in transgenosis.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully