Method for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system (variations), a device for realizing same and a method for diagnosing the defects in the hemostatic system on the basis of a change in the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system
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United States of America Patent
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Apr 10, 2018
Issued Date -
N/A
app pub date -
Jul 16, 2012
filing date -
Jul 26, 2011
priority date (Note) -
In Force
status (Latency Note)
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Abstract
The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme. The device for the implementation of the above method comprises an in vitro system, a means for illuminating the sample, a recording means and a control means. A method for diagnosing homeostatic imbalances according to a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a blood sample is also proposed.
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International Classification(s)
- 2012 Application Filing Year
- C12Q Class
- 2418 Applications Filed
- 1732 Patents Issued To-Date
- 71.63 % Issued To-Date
Cited Art Landscape
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- 0 Citation Count
- C12Q Class
- 0 % this patent is cited more than
- 7 Age
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