Sample preparation for flow cytometry
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United States of America Patent
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Apr 3, 2018
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app pub date -
Apr 4, 2013
filing date -
Apr 5, 2012
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Abstract
Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
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