Method for detecting ligand using FRET biosensor
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United States of America Patent
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Mar 20, 2018
Grant Date -
Apr 5, 2012
app pub date -
Apr 27, 2010
filing date -
Apr 27, 2009
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Abstract
The present application relates to a method for detecting ligand using a biosensor applied the FRET (fluorescence resonance energy transfer) phenomenon. More particularly, the method may be used for simply detecting a ligand in a sample by measuring the FRET of a biosensor under the conditions in which a specific critical temperature is maintained. The method may use a phenomenon in which a ligand-binding protein in a biosensor shows reversible unfolding at a temperature higher than the specific critical temperature and the level of the unfolding changes depending on the concentration of a ligand. The method can be widely applied to a variety of kinds of FRET biosensors using the ligand-binding protein.
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