Method for producing aniline derivative by fermentation from carbon source
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United States of America Patent
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Mar 20, 2018
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Sep 14, 2016
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Mar 20, 2014
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Abstract
Provided is a method for producing an aniline derivative by fermentation from a carbon source such as glucose. The method comprises the following steps: production of microorganisms capable of producing 1.8 g/L or more of 4-aminophenylalanine (4APhe) under prescribed culture conditions by introducing at least three exogenous genes into microorganisms having the ability to biosynthesize 4-aminophenylpyruvic acid from chorismic acid; and production of at least one aniline derivative selected from the group consisting of 4-aminophenylalanine (4APhe), 4-aminocinnamic acid (4ACA), 2-(4-aminophenyl)aldehyde, 4-aminophenylacetic acid, and 4-aminophenethylethanol (4APE) by bringing these microorganisms into contact with a carbon source under conditions suited to the growth and/or maintenance of these microorganisms.
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