In vitro evolution in microfluidic systems
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United States of America Patent
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Mar 20, 2018
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app pub date -
May 5, 2017
filing date -
Oct 8, 2004
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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International Classification(s)
- C12Q:MEASURING OR TESTING PROCESSES INVOLVING ENZYMES O....
- C12P:FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESI....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- B01L:CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENE....
- B01J:CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, CO....
- B01F:MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
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