Method of generating droplets
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United States of America Patent
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Feb 27, 2018
Grant Date -
May 25, 2017
app pub date -
Nov 30, 2016
filing date -
Sep 23, 2008
priority date (Note) -
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status (Latency Note)
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Abstract
Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
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International Classification(s)
- C12Q:MEASURING OR TESTING PROCESSES INVOLVING ENZYMES O....
- B29C:SHAPING OR JOINING OF PLASTICS; SHAPING OF SUBSTAN....
- B01L:CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENE....
- G01N:INVESTIGATING OR ANALYSING MATERIALS BY DETERMININ....
- B01F:MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
- B29L:INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RE....
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