Use of LAMBDA-GAM protein in ribosomal display technology
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United States of America Patent
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Feb 20, 2018
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Apr 24, 2014
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Apr 25, 2013
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Abstract
Methods and systems for increasing the stability of a nucleic acid template that encodes a protein of interest in a cell free translation system or a ribosomal display reaction system are described. In some embodiments, the nucleic acid template is an RNA or mRNA. The stability of the RNA template is increased by adding the bacteriophage lambda protein Gam to the cell free extract used in the translation system. The addition of Gam protein increases the longevity of the reaction system, thereby increasing the efficiency of the ribosomal display reaction system.
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