Fused NHase with improved specific activity and stability
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United States of America Patent
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Feb 20, 2018
Grant Date -
Oct 27, 2016
app pub date -
Sep 30, 2015
filing date -
Apr 22, 2015
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Abstract
The present invention provides a fused NHase with improved specific activity and stability, which relates to the field of genetic engineering. This invention provides a method of overexpressing a fused NHase in E. coli and producing a mutant NHase with improved stability and product tolerance. The invention provides a simple, efficient and safe method of making mutant NHase, and can produce a large amount of soluble NHases in a short period. The present invention makes a contribution to large-scale industrial production and further theoretical study of NHases.
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