Method for detecting a nucleic acid
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United States of America Patent
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Feb 13, 2018
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app pub date -
Jul 21, 2016
filing date -
Aug 3, 2015
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Abstract
A method for detecting at least one nucleic acid in a solution, comprising the steps of: providing at least one dye, wherein the solution and/or a reaction vessel, wherein the solution is present, comprises the dye and wherein the at least one dye is adapted to emit emission light due to an optical excitation by excitation light; providing at least one absorber in the solution, wherein the absorber is adapted to cause an attenuation of the emission light and/or the excitation light and wherein the attenuation is influenced by a bonding of the at least one absorber to the nucleic acid; and radiating excitation light into the solution and measuring an intensity of the emission light; wherein the attenuation of the emission light and/or the excitation light by the at least one absorber occurs independently of a quantum yield of the at least one dye.
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