Multisort cell separation method

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United States of America Patent

PATENT NO 9885032
SERIAL NO

14944991

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Abstract

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The invention is directed to a method for enriching target cells from a sample of cells characterized by:

    a) contacting the sample with a cell aggregation agent and first magnetic particles having an iron content of 0.1 pg to 5000 pg, coupled to a first antigen recognizing moiety; and second magnetic particles having an iron content of 0.05 fg to 100 fg and coupled to a second antigen recognizing moiety to obtain mixture a)b) applying a first magnetic field gradient to the mixture a) thereby removing the cells bound to the first antigen recognizing moiety coupled to the first magnetic particles, to obtain a mixture b) and obtaining an agglomerate comprising the cells of mixture a) bound to the cell aggregation agentc) applying a second magnetic field gradient to the mixture b) thereby immobilizing the cells bound to the second antigen recognizing moietyd) recovering the immobilized cells from the second magnetic field gradient as target cells.

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Patent Owner(s)

Patent OwnerAddress
MILTENYI BIOTEC B V & CO KGBERGISCH GLADBACH

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dose, Christian Kurten, DE 17 56
Huppert, Volker Kürten, DE 10 35
Kauling, Burgund Bergisch Gladbach, DE 2 17
Steinbrück, Philipp Solingen, DE 2 13

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