Process for the production of an enzymatic cocktail using solid residues from a process for the biochemical coversion of lignocellulosic materials
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United States of America Patent
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Feb 6, 2018
Grant Date -
Nov 27, 2014
app pub date -
Nov 27, 2012
filing date -
Dec 14, 2011
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Abstract
The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases:
- a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L;a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.
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