Constructs and methods for genome editing and genetic engineering of fungi and protists
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United States of America Patent
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Jan 30, 2018
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Aug 14, 2015
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Aug 15, 2014
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Abstract
Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
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