Kits to assay kinase activity
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United States of America Patent
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Jan 23, 2018
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app pub date -
Feb 2, 2016
filing date -
Feb 13, 2006
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Abstract
Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes. Kits may include an element tag for directly tagging an affinity product that recognizes phosphorylated substrates, as well as a non-phosphorylated substrate or an expression plasmid.
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