Method for fragmenting genomic DNA using CAS9
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United States of America Patent
Stats
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Jan 23, 2018
Issued Date -
N/A
app pub date -
May 29, 2014
filing date -
May 29, 2013
priority date (Note) -
In Force
status (Latency Note)
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Abstract
A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided.
First Claim
Other Claims data not available
Family
| Country | kind | publication No. | Filing Date |
|---|---|---|---|
| US | B2 | US11414695 | May 29, 2014 |
| US | A1 | US20170107560 | Oct 26, 2016 |
| US | B2 | US10577644 | Oct 31, 2016 |
| US | A1 | US20200172959 | Jan 21, 2020 |
International Classification(s)
- 2014 Application Filing Year
- C12Q Class
- 3114 Applications Filed
- 2141 Patents Issued To-Date
- 68.76 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 44 Citation Count
- C12Q Class
- 99.26 % this patent is cited more than
- 7 Age
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