RNA-mediated gene assembly from DNA oligonucleotides

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United States of America Patent

PATENT NO 9873900
APP PUB NO 20130225446A1
SERIAL NO

13763009

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Abstract

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The present invention is directed to methods and materials for RNA-mediated gene assembly from oligonucleotide sequences. In some embodiments, the oligonucleotides used for gene assembly are provided in an array format. An RNA polymerase promoter is appended to surface-bound oligonucleotides and a plurality of RNA copies of each oligonucleotide are then produced with an RNA polymerase. These RNA molecules self-assemble into a desired full-length RNA transcript by hybridization and ligation. The resulting RNA transcript may then be converted into double strand DNA useful in a variety of applications including protein expression.

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WISCONSIN ALUMNI RESEARCH FOUNDATION614 WALNUT STREET 13TH FLOOR MADISON WI 53726

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  • 2013 Application Filing Year
  • C12P Class
  • 1593 Applications Filed
  • 1388 Patents Issued To-Date
  • 87.14 % Issued To-Date
Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances2013201420152016201720182019202020212022202320240255075100

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Inventor Name Address # of filed Patents Total Citations
Smith, Lloyd Madison, US 14 206
Wu, Cheng-Hsien Madison, US 195 5583

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Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges1277890732173101 - 1011 - 2021 - 3031 - 4051 - 6071 - 80010020030040050060070080090010001100120013001400

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