Assay for detecting closely-related serotypes of human papillomavirus (HPV)
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United States of America Patent
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-
Jan 16, 2018
Issued Date -
N/A
app pub date -
Feb 12, 2014
filing date -
Feb 16, 2010
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.
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International Classification(s)
- 2014 Application Filing Year
- C12Q Class
- 3114 Applications Filed
- 2141 Patents Issued To-Date
- 68.76 % Issued To-Date
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- 0 Citation Count
- C12Q Class
- 0 % this patent is cited more than
- 7 Age
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