Cell culturing method, cell culturing apparatus and kit comprising a porous polyimide film
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United States of America Patent
Stats
-
Jan 16, 2018
Issued Date -
N/A
app pub date -
Jul 25, 2014
filing date -
Jul 26, 2013
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The present invention relates to a cell culturing method and cell culturing apparatus and kit for use therewith. The method includes applying cells on a porous polyimide film and culturing. One embodiment includes a process for sowing cells on the surface of the film. Another embodiment includes a process for placing a cell suspension on the dried surface of the film and leaving the film undisturbed, moving the film to promote liquid efflux, or stimulating a portion of the surface to entangle the cell suspension into the film, and then retaining the cells in suspension inside the film while allowing moisture to flow out. Another embodiment includes a process for moistening one or both surfaces of the film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened film, retaining the cells in suspension inside the film, and allowing moisture to flow out.
First Claim
Other Claims data not available
Family
International Classification(s)
- 2014 Application Filing Year
- C12N Class
- 5730 Applications Filed
- 4275 Patents Issued To-Date
- 74.61 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 0 Citation Count
- C12N Class
- 0 % this patent is cited more than
- 7 Age
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | Jul 16, 2025 |
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Jul 16, 2029 |
