Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases
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United States of America Patent
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Jan 9, 2018
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May 25, 2016
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Jun 10, 2011
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Abstract
The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.
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