Method for nucleotide detection
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United States of America Patent
Stats
-
Jan 2, 2018
Issued Date -
N/A
app pub date -
Jun 3, 2015
filing date -
Jan 31, 2011
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.
First Claim
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International Classification(s)
- 2015 Application Filing Year
- C12Q Class
- 3483 Applications Filed
- 2244 Patents Issued To-Date
- 64.43 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 2 Citation Count
- C12Q Class
- 57.71 % this patent is cited more than
- 7 Age
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