Method for increasing the secretion of recombinant proteins
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United States of America Patent
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Dec 26, 2017
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Apr 17, 2013
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Apr 17, 2012
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Abstract
The invention relates to a method for producing a recombinant protein in cells with a cell wall, comprising the step of increasing the secretion of the recombinant protein through the cell wall by expression of the protein in the cells in a culture medium containing a combination of a surface-active polymer and monovalent metal ions and with an osmolarity at least 0.32 osmol/L, said invention further relating to culture media and nutrient mixtures for the method.
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