Alkaline feed
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United States of America Patent
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Dec 19, 2017
Grant Date -
May 14, 2015
app pub date -
Jan 28, 2015
filing date -
Aug 30, 2010
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Abstract
A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide.
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