Methods for analyzing DNA
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United States of America Patent
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Dec 12, 2017
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app pub date -
Oct 21, 2013
filing date -
Oct 22, 2012
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Abstract
The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.
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