In vitro method for assessing cytokine storm responses
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United States of America Patent
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Dec 5, 2017
Grant Date -
Feb 18, 2016
app pub date -
Mar 21, 2014
filing date -
Mar 22, 2013
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Abstract
The present disclosure an in vitro method of assaying the stimulation of a cytokine storm response comprising the steps of: a. co-culturing PBMCs and matched differentiated endothelial cells to provide a system representative of human responses in vivo, and b. exposing the co-cultured cell system to a test agent, c. analyzing the system for the presence of one or more cytokines released after exposing the co-culture system to said test agent, and d. optionally evaluating the response to the test agent in comparison to a response to one or more control agents.
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