Amplification and detection of ribonucleic acids
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United States of America Patent
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Dec 5, 2017
Issued Date -
N/A
app pub date -
Mar 6, 2017
filing date -
Jan 14, 2008
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
Compositions, methods, and kits for detecting one or more species of RNA molecules are disclosed. In one embodiment, a first adaptor and a second adaptor are ligated to the RNA molecule using a polypeptide comprising double-strand specific RNA ligase activity, without an intervening purification step. The ligated product is reverse transcribed, then at least some of the ribonucleosides in the reverse transcription product are removed. Primers are added and amplified products are generated. In certain embodiments, the sequence of at least part of at least one species of amplified product is determined and at least part of the corresponding RNA molecule is determined. In some embodiments, at least some of the amplified product species are detected, directly or indirectly, allowing the presence and/or quantity of the RNA molecule of interest to be determined.
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Cited Art Landscape
Patent Citation Ranking
- 3 Citation Count
- C12Q Class
- 52.11 % this patent is cited more than
- 8 Age
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|---|---|---|---|---|
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | Jun 5, 2025 |
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