Method for detecting mutant DNA
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United States of America Patent
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Dec 5, 2017
Issued Date -
N/A
app pub date -
Sep 22, 2011
filing date -
Sep 22, 2010
priority date (Note) -
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status (Latency Note)
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Abstract
The present invention relates to a method for detecting of a mutant DNA using a probe, comprising:
- (1) contacting a sample containing a single-stranded DNA which has a substituted nucleotide, a deleted nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA (wild-type DNA) corresponding thereto with the probe which hybridizes with both single-stranded DNA, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with a wild-type DNA (wild-type hybrid), wherein at least one of the obtained mutant-type hybrid and wild-type hybrid has the stem structure;(2) separating the obtained mutant-type hybrid or/and wild-type hybrid by electrophoresis on the basis of presence or absence of the stem structure or difference in the stem structure; and(3) detecting the presence or absence of the mutant-type DNA in the sample.
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International Classification(s)
- 2011 Application Filing Year
- C07H Class
- 1856 Applications Filed
- 1075 Patents Issued To-Date
- 57.93 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 0 Citation Count
- C07H Class
- 0 % this patent is cited more than
- 8 Age
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | Jun 5, 2025 |
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Jun 5, 2029 |
