Transposon nucleic acids comprising a calibration sequence for DNA sequencing
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United States of America Patent
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Dec 5, 2017
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Aug 26, 2015
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Jul 20, 2011
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Abstract
Transposon nucleic acids comprising a transposon end sequence and a calibration sequence for DNA sequencing in the transposon end sequence. In one embodiment, the transposon end sequence is a Mu transposon end. A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with the calibration sequence providing facilitated downstream handling of the produced DNA fragments, e.g., in the generation of sequencing templates.
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