CRISPR-Cas component systems, methods and compositions for sequence manipulation
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United States of America Patent
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Nov 21, 2017
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Jan 7, 2016
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Dec 12, 2012
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Abstract
The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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