Blastoid, cell line based artificial blastocyst
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United States of America Patent
Stats
-
Nov 21, 2017
Issued Date -
N/A
app pub date -
Apr 16, 2014
filing date -
Apr 16, 2013
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A method for making an at least double layered cell aggregate and/or an artificial blastocyst, and/or a further-developed blastoid termed blastoid, by forming a double layered cell aggregate from at least one trophoblast cell and at least one pluripotent and/or totipotent cell, and culturing the aggregate to obtain an artificial blastocyst having a trophectoderm-like tissue that surrounds a blastocoel and an inner cell mass-like tissue. The cell aggregate can be formed from toti- or pluripotent stem cell types, or induced pluripotent stem cell types, in combination with trophoblast stem cells. Formation of a blastoid can be achieved by culturing the cell aggregate in a medium preferably comprising one or more of a Rho/ROCK inhibitor, a Wnt pathway modulator, a PKA pathway modulator, a PKC pathway modulator, a MAPK pathway modulator, a STAT pathway modulator, an Akt pathway modulator, a Tgf pathway modulator and a Hippo pathway modulator. Also, a method for growing an at least double layered cell aggregate into an artificial blastocyst, and into a further-developed blastoid, a foetus or a live animal and an in vitro cell culture comprising the mentioned compounds and/or cell aggregates.
First Claim
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International Classification(s)
- 2014 Application Filing Year
- C12N Class
- 5730 Applications Filed
- 4275 Patents Issued To-Date
- 74.61 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 0 Citation Count
- C12N Class
- 0 % this patent is cited more than
- 8 Age
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|---|---|---|---|---|
| 7.5 Year Payment | $3600.00 | $1800.00 | $900.00 | May 21, 2025 |
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | May 21, 2029 |
