Methods for obtaining liquid from a solid phase

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United States of America Patent

PATENT NO 9821248
SERIAL NO

13834901

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Abstract

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A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.

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Patent Owner(s)

Patent OwnerAddress
CAMBRIDGE ENTERPRISE LIMITEDTHE OLD SCHOOLS TRINITY LANE CAMBRIDGE CB2 1TN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chua, Yii Leng Cambridge, GB 5 8

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