In situ restoration of apatite-based chromatography resins
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United States of America Patent
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Nov 14, 2017
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May 10, 2013
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May 30, 2012
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Abstract
Methods and compositions are provided for treatment of an apatite-based resin from which retained solutes have been eluted by an elution buffer that contains an alkali metal salt with solutions of calcium ion, phosphate ion, and hydroxide separately from any sample loading and elution buffers. The treatment solutions restore the resin, reversing the deterioration that is caused by the alkali metal salt in the elution buffer.
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