Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate
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United States of America Patent
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Oct 31, 2017
Grant Date -
Jan 6, 2011
app pub date -
Oct 14, 2008
filing date -
Oct 15, 2007
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Abstract
The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly taurine transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.
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