Methods for transforming tarwi and for producing molecular farming products in transgenic tarwi seed

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United States of America Patent

PATENT NO 9796981
SERIAL NO

14056297

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Abstract

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The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.

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Patent Owner(s)

Patent OwnerAddress
NATIONAL RESEARCH COUNCIL OF CANADA1200 MONTREAL ROAD OTTAWA K1A 0R6

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  • 2013 Application Filing Year
  • C12N Class
  • 5078 Applications Filed
  • 4006 Patents Issued To-Date
  • 78.89 % Issued To-Date
Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances20132014201520162017201820192020202120222023202420250255075100

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Doshi, Ketan M Saskatoon, CA 2 0
Holbrook, Larry Saskatoon, CA 1 0
Loukanina, Natalia N Saskatoon, CA 1 0
Pollock, Brent Saskatoon, CA 1 0
Polowick, Patricia Saskatoon, CA 1 0

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