Cooling systems and methods for cryo super-resolution fluorescence light microscopy and other applications
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United States of America Patent
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Oct 10, 2017
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Sep 13, 2013
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Sep 13, 2012
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Abstract
New systems and methods are described for maintaining a desired steady state temperature differential between two objects that may otherwise undergo heat transfer to restore thermal steady state. In one application, a cooling microscope assembly and its use with conventional optical microscopes are described for achieving super-resolution imaging. The assembly allows for the high resolution imaging of samples at cryogenic temperatures while maintaining the temperature of the objective lens above freezing by employing circulation systems and a coupling fluid between the sample and objective Jens.
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