Labeled chiral alpha-hydroxy ketoacid derivatives, a process for preparing said derivatives and their use
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United States of America Patent
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Oct 10, 2017
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Jul 30, 2015
app pub date -
Aug 7, 2013
filing date -
Aug 8, 2012
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Abstract
The present invention relates to labeled chiral alpha-hydroxy ketoacid derivatives, a process for preparing the derivatives and their use for isotopic labeling of amino acids, in particular, for isotopic labeling of methyl groups of amino acids, and more particularly, for specific isotopic labeling of valine, leucine and isoleucine methyl groups, in proteins and biomolecular assemblies. The invention also concerns a process for analyzing proteins and biomolecular assemblies by NMR spectroscopy including a step of isotopic labeling of amino acids, in particular, valine, leucine and isoleucine, in proteins and biomolecular assemblies to be analyzed by the chiral alpha-hydroxy ketoacid derivatives of the invention.
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