Method for separation of monomeric polypeptides from aggregated polypeptides
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United States of America Patent
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Oct 10, 2017
Grant Date -
May 22, 2014
app pub date -
Jun 28, 2012
filing date -
Jul 1, 2011
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Abstract
Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide m monomeric form and m aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide m monomeric form.
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