Amplification of complex nucleic acids

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United States of America Patent

PATENT NO 9777316
SERIAL NO

13375046

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Abstract

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What is described is a method for quantitative and qualitative analysis of complex template nucleic acids to be analyzed. The method comprises the co-amplification of a control nucleic acid with the complex template nucleic acid by means of isothermal strand displacement reaction. The method of the invention further comprises the determination of the amount of amplified control nucleic acid as measure for the determination of the quantity and/or quality of the complex template nucleic acid used. The present invention also relates to a kit for carrying out a method of the invention. Furthermore, the use of the method of the invention or the kit of the invention for standardization of whole-genome, whole-transcriptome and whole-bisulfitome analyses is described.

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Patent Owner(s)

Patent OwnerAddress
QIAGEN GMBH40724 HILDEN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Fisch, Evelyn Hilden, DE 1 1
Korfhage, Christian Langenfeld, DE 35 385

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