Organic small hairpin RNAs
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United States of America Patent
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Oct 3, 2017
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Jan 29, 2014
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May 31, 2013
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Abstract
Disclosed are improved shRNA molecules, termed “organic shRNA” (OshRNA), that incorporate certain structural features that increase the likelihood that the desired guide strand is produced while reducing accumulation of passenger strands that might contribute to off-target effects. Also provided herein are nucleic acids encoding OshRNAs, kits, cells, and transgenic animals comprising such nucleic acids, as well as methods of making and using OshRNAs and/or nucleic acids encoding OshRNAs.
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