Retroviral transduction using poloxamers
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United States of America Patent
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Sep 26, 2017
Grant Date -
Mar 5, 2015
app pub date -
Feb 28, 2013
filing date -
Feb 29, 2012
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Abstract
The present invention relates to a method for transducing a target cell, the method comprising the step of contacting a target cell with a retroviral vector and a poloxamer having a molecular weight of 12.8 kDa to about 15 kDa. Further, the invention relates to the use of a poloxamer as defined herein, optionally in combination with a polycationic substance as defined herein, for transducing a target cell with a retroviral vector and a kit comprising a retroviral vector, a poloxamer as defined herein and, optionally, instructions for use.
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